Forum Lyme Francophone

1: Brorson O et al. Destruction of spirochete Bor...[PMID: 19843691]
PMID- 19843691
OWN - NLM
STAT- Publisher
DA - 20091021
IS - 1091-6490 (Electronic)
DP - 2009 Oct 20
TI - Destruction of spirochete Borrelia burgdorferi round-body propagules (RBs) by the
antibiotic Tigecycline.
AB - Persistence of tissue spirochetes of Borrelia burgdorferi as helices and round
bodies (RBs) explains many erythema-Lyme disease symptoms. Spirochete RBs
(reproductive propagules also called coccoid bodies, globular bodies, spherical
bodies, granules, cysts, L-forms, sphaeroplasts, or vesicles) are induced by
environmental conditions unfavorable for growth. Viable, they grow, move and
reversibly convert into motile helices. Reversible pleiomorphy was recorded in at
least six spirochete genera (>12 species). Penicillin solution is one unfavorable
condition that induces RBs. This antibiotic that inhibits bacterial cell wall
synthesis cures neither the second "Great Imitator" (Lyme borreliosis) nor the
first: syphilis. Molecular-microscopic techniques, in principle, can detect in
animals (insects, ticks, and mammals, including patients) helices and RBs of live
spirochetes. Genome sequences of B. burgdorferi and Treponema pallidum
spirochetes show absence of >75% of genes in comparison with their free-living
relatives. Irreversible integration of spirochetes at behavioral, metabolic, gene
product and genetic levels into animal tissue has been documented. Irreversible
integration of spirochetes may severely impair immunological response such that
they persist undetected in tissue. We report in vitro inhibition and destruction
of B. burgdorferi (helices, RBs = "cysts") by the antibiotic Tigecycline (TG;
Wyeth), a glycylcycline protein-synthesis inhibitor (of both 30S and 70S ribosome
subunits). Studies of the pleiomorphic life history stages in response to TG of
both B. burgdorferi and Treponema pallidum in vivo and in vitro are strongly
encouraged.
AD - Department of Microbiology, Sentralsykehuset i Vestfold HF, N-3116 Tonsberg,
Norway.
AU - Brorson O
AU - Brorson SH
AU - Scythes J
AU - Macallister J
AU - Wier A
AU - Margulis L
LA - ENG
PT - JOURNAL ARTICLE
DEP - 20091020
TA - Proc Natl Acad Sci U S A
JT - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
EDAT- 2009/10/22 06:00
MHDA- 2009/10/22 06:00
CRDT- 2009/10/22 06:00
AID - 0908236106 [pii]
AID - 10.1073/pnas.0908236106 [doi]
PST - aheadofprint
SO - Proc Natl Acad Sci U S A. 2009 Oct 20.

PMID- 19995919
OWN - NLM
STAT- MEDLINE
DA - 20100125
DCOM- 20100304
IS - 1098-6596 (Electronic)
IS - 0066-4804 (Linking)
VI - 54
IP - 2
DP - 2010 Feb
TI - Ineffectiveness of tigecycline against persistent Borrelia burgdorferi.
PG - 643-51
AB - The effectiveness of a new first-in-class antibiotic, tigecycline
(glycylcycline), was evaluated during the early dissemination (1 week), early
immune (3 weeks), or late persistent (4 months) phases of Borrelia burgdorferi
infection in C3H mice. Mice were treated with high or low doses of tigecycline,
saline (negative-effect controls), or a previously published regimen of
ceftriaxone (positive-effect controls). Infection status was assessed at 3 months
after treatment by culture, quantitative ospA real-time PCR, and subcutaneous
transplantation of joint and heart tissue into SCID mice. Tissues from all
saline-treated mice were culture and ospA PCR positive, tissues from all
antibiotic-treated mice were culture negative, and some of the tissues from most
of the mice treated with antibiotics were ospA PCR positive, although the DNA
marker load was markedly decreased compared to that in saline-treated mice.
Antibiotic treatment during the early stage of infection appeared to be more
effective than treatment that began during later stages of infection. The
viability of noncultivable spirochetes in antibiotic-treated mice (demonstrable
by PCR) was confirmed by transplantation of tissue allografts from treated mice
into SCID mice, with dissemination of spirochetal DNA to multiple recipient
tissues, and by xenodiagnosis, including acquisition by ticks, transmission by
ticks to SCID mice, and survival through molting into nymphs and then into
adults. Furthermore, PCR-positive heart base tissue from antibiotic-treated mice
revealed RNA transcription of several B. burgdorferi genes. These results
extended previous studies with ceftriaxone, indicating that antibiotic treatment
is unable to clear persisting spirochetes, which remain viable and infectious,
but are nondividing or slowly dividing.
AD - Center for Comparative Medicine, School of Medicine, University of California at
Davis, One Shields Avenue, Davis, CA 95616, USA. swbarthold@ucdavis.edu
FAU - Barthold, Stephen W
AU - Barthold SW
FAU - Hodzic, Emir
AU - Hodzic E
FAU - Imai, Denise M
AU - Imai DM
FAU - Feng, Sunlian
AU - Feng S
FAU - Yang, Xiaohua
AU - Yang X
FAU - Luft, Benjamin J
AU - Luft BJ
LA - eng
GR - AI-26815/AI/NIAID NIH HHS/United States
PT - Journal Article
PT - Research Support, N.I.H., Extramural
PT - Research Support, Non-U.S. Gov't
DEP - 20091207
PL - United States
TA - Antimicrob Agents Chemother