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Nelly
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Vaccin anti-tique (à suivre)

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Protective immunity against Ixodes ricinus induced by a salivary serpin

P.-P. Prevot, B. Couvreur, V. Denis, M. Brossard, L. Vanhammea, 1 and E. Godfroid, 1, ,
aInstitut de Biologie et de Médecine Moléculaires, Université Libre de Bruxelles, Rue des Professeurs Jeener et Brachet, 12, B-6041 Gosselies, Belgium
bLaboratoire de Parasitologie, Département de Biologie, Université de Neuchâtel, CH-2007 Neuchâtel, Switzerland
Received 20 October 2006; revised 18 December 2006; accepted 4 January 2007. Available online 16 January 2007.


Abstract

Iris is a specific elastase inhibitor expressed in the salivary glands of the hard tick Ixodes ricinus. It belongs to the superfamily of serpins and interferes with both haemostasis and the immune response of the host. In this study, we first show that Iris is expressed in nymphs but not in the female midgut nor in males. We also show that Iris is present in the saliva. To examine its potency as anti-tick vaccine candidate, we set up three models of I. ricinus infestation on immunized animals: nymphs on mice, and adults and nymphs on rabbits. We report the rise of neutralizing antibodies following immunization of rabbits and mice. This comes with a significant protective immunity against ticks in rabbits only, resulting in a 30% mortality rate and a diminution of weight gain in both nymphs and adults and a prolongation of blood feeding time in adults. This is the first report on an anti-tick vaccine trial on I. ricinus using a protein able to interact with both host immunity and haemostasis, as a vaccinating antigen.

Ceci est le premier rapport sur un éssai de vaccin anti-tique sur I.ricinus qui utilise une protéine capable d'interagir avec à la fois l'immunité et l'hémostase de l'hôte, en tant qu'antigène vaccinal.

Keywords: Tick; Serpin; Ixodes ricinus


Corresponding author. Tel.: +32 2 6509934; fax: +32 2 6509900.
1 They share the senior authorship of this work.

Vaccine
Volume 25, Issue 17, 30 April 2007, Pages 3284-3292
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